[iHuman Seminar] Metabolically-regulated organization of 26S Proteasomes in situ

ON2024-05-27TAG: ShanghaiTech UniversityCATEGORY: Lecture

Topic: Metabolically-regulated organization of 26S Proteasomes in situ

Speaker: Dr. Tang Xiaomeng, University Medical Center Göttingen (UMG)

Date and time: May 28, 16:00–17:00

Venue: Auditorium, Y Building

Host: Wolfgang Baumeister


Abstract:

The proteasome, a multi-subunit proteolytic machine, plays a crucial role in cellular homeostasis by eliminating misassembled, dysfunctional, and surplus proteins. Previous studies have revealed that proteasomes can aggregate into storage granules (PSGs) when glucose is depleted. However, despite biochemical exploration, the enigmatic structure of PSGs remains elusive. In our research, we employ state-of-the-art Cryo-electron tomography (Cryo-ET) to uncover the in situ structure of PSGs. Our findings reveal that proteasome 26S trimers are stored in a paracrystalline arrangement. Additionally, we depict the PSG formation process, transitioning from a proliferating state to a quiescent state. 

I will introduce the Cryo-ET workflow and highlight several new optional techniques. Additionally, I will discuss strategies for faster data collection in transmission electron microscopy (TEM).


Biography:

  • 2022-2024, Postdoc Research Fellow; University Medical Center Göttingen, Göttingen, Germany 

  • 2019-2022, Postdoc Research Fellow; Max-Planck-Institute of Biochemistry, Martinsried, Germany 

  • 2017-2019, Visiting Scholar; iHmuan Institute, ShanghaiTech University, Shanghai, China

  • 2012-2017, Ph.D., Biological Chemistry and Molecular Biology, Institute of Biophysics, Chinese Academy of Sciences, China