Topic: Structure of membrane proteins in native membranes by cryo-EM
Speaker: Dr. Misha Kudryashev, Max Delbrück Centre for Molecular Medicine in the Helmholtz Society (MDC-Berlin)
Date and time: September 11, 15:00–16:00
Venue: Auditorium, Y Building
Host: Wolfgang Baumeister
Abstract:
Subtomogram averaging from cryo-ET is a powerful method to determine structures of macromolecules in their native state. For macromolecules such as membrane proteins, which are present in tomograms in limited numbers, the throughput of data processing and the processing time are bottlenecks in obtaining high-resolution reconstructions. I will introduce the tools that we developed in our lab for in situ structural biology with a focus on a large ion channel RyR1 which is a part of the excitation-contraction coupling in muscle. TomoBEAR is a workflow for the processing of tomographic data utilizing common cryo-EM tools and original code allowing transparent near-automated tomographic data preprocessing, alignment, reconstruction, and particle identification followed by structural analysis. In the second part of the I will show our recent results on understanding the molecular architecture of synaptic vesicles. We imaged neurons grown on EM grids and purified synaptic vesicles by cryo-ET. We could identify individual proteins important for the function of synaptic vesicles.
Biography:
2021-present
Group Leader, Max Delbrück Centre for Molecular Medicine in the Helmholtz Society, Berlin, Germany
2022-present
Co-appointment as a W2 Professor for In Situ Structural Biology, Institute of Medical Physics and Biophysics of Charité-Universitätsmedizin, Berlin, Germany
2009-2015
Postdoc, Biozentrum, University of Basel, Switzerland
2005-2019
PhD, University of Heidelberg in collaboration with the Max Planck Institute for Biochemistry