Topic: Developing structural interactomics and its application in structural biology
Speaker: Dr. Liu Fan, Structural Interactomics Leibniz-Forschungsinstitut for Molecular Pharmacology (Leibniz-FMP)
Date and time: September 11, 14:00–15:00
Venue: Auditorium, Y Building
Host: Wolfgang Baumeister
Abstract:
Cross-linking mass spectrometry (XL-MS) is a powerful approach that can reveal thousands of protein-protein interactions (PPls) together with their sub-domain level binding interfaces. Here, we combine XL-MS, AlphaFold2 and cryoEM to obtain system-level structural insights of a giant virus family member, Melbournevirus. We obtained a virion-wide PPI map consisting of 328 direct protein-protein associations based on 7700 cross-links and resolved the localization of many viral proteins into sub-virion compartments. Further, we used AlphaFold2 to predict structures of cross-linked proteins and multimeric complexes and used the cross-linking data to validate individual high-scoring predictions which resulted in 37 predicted models. We assigned additional components of the capsid in the cryoEM map and proposed functions of uncharacterized Melbournevirus proteins. Our results provide valuable resources for better understanding of Melbournevirus and related giant viruses, also represent a blueprint for an integrated workflow for structural interactome investigation of poorly characterized viruses and beyond.
Biography:
2017-Present
Group Leader and head of the proteomics research platformLeibniz-Forschungsinstitut for Molecular Pharmacology, Berlin
2013-2017
Postdoctoral in the lab of Prof. Albert HeckUtrecht, The Netherlands
2007-2013
PhD in Biochemistry in the lab of Prof. Mike GosheNorth Carolina State University